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rabbit monoclonal anti-darpp32  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit monoclonal anti-darpp32
    Rabbit Monoclonal Anti Darpp32, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 95/100, based on 222 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/anti+darpp32/DARPP-32+Rabbit+mAb/pmc12876317-5-0-7
    Average 95 stars, based on 222 article reviews
    rabbit monoclonal anti-darpp32 - by Bioz Stars, 2026-09
    95/100 stars

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    Related Articles

    Staining:

    Article Title: Generation of human striatal organoids and cortico-striatal assembloids from human pluripotent stem cells
    Article Snippet: .. The following primary antibodies were used for staining: anti-Calbindin (Synaptic Systems, #214 011, 1:200, Lot# 214011/5), anti-Calretinin (rabbit, SWANT, CR7697, 1:200), anti-MAP2 (guinea pig, Synaptic Systems 188 004, 1:200 dilution, Lot# 2–26), anti-GFAP (rabbit, DAKO, Z0334, 1:1000 dilution, Lot#20035993), anti-MBP (rat, Millipore, MAB386, 1:300 dilution), anti-ASCL1 (mouse, BD, 556604, 1:200 dilution, Lot# 6063825), anti-FOXP2 (mouse, Santa Cruz Biotechnology, sc-517261, 1:100 dilution, Lot# 1517), anti-GAD65 (goat, R&D systems, AF2247, 1:100 dilution, Lot# UCX0218101), anti-CTIP2 (rat, Abcam, ab18465 1:300 dilution, Lot# GR322373–6, Lot# GR3272266–4), anti-DARPP32 (Cell Signaling, 2306, 1:200 dilution, Lot# 7), anti-DARPP32 (rabbit, abcam, #ab40801, 1:200, Lot#GR3213231–3), anti-NeuN (mouse, abcam, ab104224, 1:200, Lot#GR3341933–1), anti-GAD67 (mouse, Millipore Sigma, MAB5406, 1:200 dilution, Lot# 2676521), anti-GFP (chicken, GeneTex, GTX13970, 1:1000 dilution, Lot# 821905323, Lot# 821704840, Lot# 821805508), anti-DRD2 (rabbit, MBL, #MC-1405, 1:200, Lot#107522), anti-mCherry (rabbit, GeneTex, GTX128508, 1:500 dilution, Lot# 42025), anti-PSD95 (guinea pig, Invitrogen, #MA1–045, 1:200, Lot#SC249676), anti-SATB2 (mouse, Abcam, ab51502, 1:50 dilution, Lot# GR178264–4), anti-OCT4 (C30A3) (rabbit, Cell Signaling Technology, #2840, 1:200 dilution, Lot# 9), anti-SSEA4 (MC813) (mouse, Cell Signaling Technology, #4755, 1:200 dilution, Lot# 4). .. Alexa Fluor dyes, donkey anti-rabbit IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor 488 (Thermo Fisher SCIENTIFIC, A-21206, 1:1000 dilution), donkey anti-mouse IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor 568 (Thermo Fisher SCIENTIFIC, A10037, 1:1000 dilution, Lot#2110843), donkey anti-tabbit IgG (H+L) h highly cross-adsorbed secondary antibody, Alexa Fluor 568 (Thermo Fisher SCIENTIFIC, A10042, 1:1000 dilution, Lot#2136776), donkey anti-mouse IgG (H+L) H highly cross-adsorbed secondary antibody, Alexa Fluor 647 (Thermo Fisher SCIENTIFIC, A-31571, 1:1000 dilution, Lot#1839633), donkey anti-goat IgG (H+L) highly cross-adsorbed secondary antibody, Alexa Fluor 647 (Thermo Fisher SCIENTIFIC, A-21447, 1:1000 dilution, Lot#1661244), Alexa Fluor® 488 AffiniPure, donkey anti-chicken IgY (IgG) (H+L) (Jackson ImmunoResearch Inc, 703-545-155, 1:1000 dilution), Alexa Fluor® 488 AffiniPure donkey anti-guinea pig IgG (H+L) (Jackson ImmunoResearch Inc, 706-545-148, 1:1000 dilution), Alexa Fluor® 647 AffiniPure donkey anti-rat IgG (H+L) (Jackson ImmunoResearch Inc, 712-605-153, 1:1000 dilution), Alexa Fluor® 647 AffiniPure donkey anti-rabbit IgG (H+L) (Jackson ImmunoResearch Inc, 711-605-152, 1:1000 dilution), Alexa Fluor® 647 AffiniPure donkey anti-rat IgG (H+L) (Jackson ImmunoResearch Inc, 712-605-153, 1:1000 dilution), Alexa Fluor® 647 AffiniPure donkey anti-guinea pig IgG (H+L) (Jackson ImmunoResearch Inc, 706-605-148, 1:1000 dilution) were used at 1:1,000 dilution, and nuclei were visualized with Hoechst 33258 (Thermo Fisher Science, H3569, 1:10,000 dilution, Lot#1829927).

    Next-Generation Sequencing:

    Article Title: Shedding a new light on Huntington's disease: how blood can both propagate and ameliorate disease pathology.
    Article Snippet: .. Detection of neurons and microglia in the striatum and cortex Free-floating sections were rinsed in 0.1 M PBS, placed in 3% H2O2 for 30 min and blocked with 5% NGS (Wisent Bioproducts, #053-150) and 0.1% Triton X-100 in PBS 0.1 M. Sections were then incubated O/N at 4 °C with one of the following antibodies in blocking buffer; anti-Iba1 (1:1000, Wako, #019-19741), anti-calbindin (1:1000, Sigma-Aldrich, #C9848) or anti-DARPP32 (1:1000, Cell signaling, #2306S). .. After primary antibody incubation, samples were washed 3 × 8 min in 0.1 M PBS and incubated for 1 h at RT in either biotinylated goat anti-mouse secondary antibody (1:1500, Vector Laboratories, #BA9200) or biotinylated goat anti-rabbit secondary antibody (1:1500, Vector Laboratories, #BA1000) diluted in blocking solution and washes were repeated.

    Incubation:

    Article Title: Shedding a new light on Huntington's disease: how blood can both propagate and ameliorate disease pathology.
    Article Snippet: .. Detection of neurons and microglia in the striatum and cortex Free-floating sections were rinsed in 0.1 M PBS, placed in 3% H2O2 for 30 min and blocked with 5% NGS (Wisent Bioproducts, #053-150) and 0.1% Triton X-100 in PBS 0.1 M. Sections were then incubated O/N at 4 °C with one of the following antibodies in blocking buffer; anti-Iba1 (1:1000, Wako, #019-19741), anti-calbindin (1:1000, Sigma-Aldrich, #C9848) or anti-DARPP32 (1:1000, Cell signaling, #2306S). .. After primary antibody incubation, samples were washed 3 × 8 min in 0.1 M PBS and incubated for 1 h at RT in either biotinylated goat anti-mouse secondary antibody (1:1500, Vector Laboratories, #BA9200) or biotinylated goat anti-rabbit secondary antibody (1:1500, Vector Laboratories, #BA1000) diluted in blocking solution and washes were repeated.

    Blocking Assay:

    Article Title: Shedding a new light on Huntington's disease: how blood can both propagate and ameliorate disease pathology.
    Article Snippet: .. Detection of neurons and microglia in the striatum and cortex Free-floating sections were rinsed in 0.1 M PBS, placed in 3% H2O2 for 30 min and blocked with 5% NGS (Wisent Bioproducts, #053-150) and 0.1% Triton X-100 in PBS 0.1 M. Sections were then incubated O/N at 4 °C with one of the following antibodies in blocking buffer; anti-Iba1 (1:1000, Wako, #019-19741), anti-calbindin (1:1000, Sigma-Aldrich, #C9848) or anti-DARPP32 (1:1000, Cell signaling, #2306S). .. After primary antibody incubation, samples were washed 3 × 8 min in 0.1 M PBS and incubated for 1 h at RT in either biotinylated goat anti-mouse secondary antibody (1:1500, Vector Laboratories, #BA9200) or biotinylated goat anti-rabbit secondary antibody (1:1500, Vector Laboratories, #BA1000) diluted in blocking solution and washes were repeated.

    Western Blot:

    Article Title: Dopamine neuron morphology and output are differentially controlled by mTORC1 and mTORC2
    Article Snippet: Antibody , Goat anti-rabbit Alexa 633 secondary (goat polyclonal) , Thermo Fisher , Cat #: A11034 , (1:500) IHC. .. Antibody , Anti-DARPP32 (rabbit monoclonal) , Cell Signaling , Cat #: 2,306 S , (1:1500) WB. .. Antibody , Anti-Histone-3 (mouse monoclonal) , Cell Signaling , Cat #: 96C10 , (1:1500) WB.

    other:

    Article Title: Directed differentiation of functional corticospinal-like neurons from endogenous SOX6+/NG2+ cortical progenitors
    Article Snippet: Antibody , Anti-DARPP32 (Rabbit polyclonal) , Cell Signaling Technology , 2306 S; RRID: AB_823479 , (1:250).



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    Image Search Results


    (a ) HD mouse model (R6/2) was crossed with CD22-knockout (KO) mice to evaluate the biological impact of CD22 in vivo. ( b ) Rotarod performance was assessed from 7 to 12 weeks of age. Knockout of CD22 significantly improved the rotarod performance of the HD mice. Data are presented as mean ± SEM (n = 9-12 animals per group). *Specific comparison between WT and HD mice in each condition; #Specific comparison between CD22-WT and CD22-KO in each condition by two-way ANOVA. Immunofluorescence staining of brain sections was performed to detect mHTT aggregates (green, EM48) ( c ) and the expression of the medium spiny neuron marker (green, Darpp32) ( e ) in the neurons (red, NeuN) of HD mice. ( d ) Quantification of (c). ( f ) Quantification of (e). Knockout of CD22 reduces the area and intensity of mHTT aggregates and the loss of Darpp32 in HD mice. Data are presented as mean ± SEM (n = 6-8 animals per group). * p < 0.05, ** or ## p < 0.01, *** p < 0.001 and **** p < 0.0001 by unpaired t -test. Scale bar = 10 µm.

    Journal: bioRxiv

    Article Title: Oxidative Stress-Induced Microglial CD22 Upregulation Impairs Phagocytosis and Exacerbates Huntington’s Disease

    doi: 10.64898/2026.03.11.710967

    Figure Lengend Snippet: (a ) HD mouse model (R6/2) was crossed with CD22-knockout (KO) mice to evaluate the biological impact of CD22 in vivo. ( b ) Rotarod performance was assessed from 7 to 12 weeks of age. Knockout of CD22 significantly improved the rotarod performance of the HD mice. Data are presented as mean ± SEM (n = 9-12 animals per group). *Specific comparison between WT and HD mice in each condition; #Specific comparison between CD22-WT and CD22-KO in each condition by two-way ANOVA. Immunofluorescence staining of brain sections was performed to detect mHTT aggregates (green, EM48) ( c ) and the expression of the medium spiny neuron marker (green, Darpp32) ( e ) in the neurons (red, NeuN) of HD mice. ( d ) Quantification of (c). ( f ) Quantification of (e). Knockout of CD22 reduces the area and intensity of mHTT aggregates and the loss of Darpp32 in HD mice. Data are presented as mean ± SEM (n = 6-8 animals per group). * p < 0.05, ** or ## p < 0.01, *** p < 0.001 and **** p < 0.0001 by unpaired t -test. Scale bar = 10 µm.

    Article Snippet: The primary antibodies included NeuN (Merck Millipore; ABN78), mEM48 (Merck Millipore; MAB5374), Darpp32 (Santa Cruz; sc-271111), Iba1 (FUJIFILM Wako Pure Chemical Corporation; 019-19741), and CD68 (Bio-Rad; MCA1957).

    Techniques: Knock-Out, In Vivo, Comparison, Immunofluorescence, Staining, Expressing, Marker